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Patient-Specific induced Pluripotent Stem Cell-Derived Hepatocyte-Like Cells as a Model to Study Autosomal Recessive Hypercholesterolemia.(No resources)

Stem cells and development. 2021-05;

Nikasa P,Tricot T,Mahdieh N,Baharvand H,Totonchi M,Hejazi MS,M Verfaillie C

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Proteins, Expression, Isolation and Analysis	… The top 3 off-target regions of sgRNA 1 and 2 were determined using the CRISPR genome editing tool from IDT. Primers were designed … Seventy µg of protein were loaded on a 4%–20% ExpressPlus PAGE Gel (Genscript), and separated via electrophoresis. Next, the …	Get A Quote